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cd16a 6his tagged recombinant  (TargetMol)


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    Structured Review

    TargetMol cd16a 6his tagged recombinant
    (A) Schematic representation of the BiKE structure. (B) Schematic representation of BiKE-mediated NK cells killing of tumors. (C) BiKE SDS-PAGE of non-reduced and reduced proteins, stained with Coomassie blue. (D) ELISA test measuring the affinity of different concentrations of BiKE to the LILRB4 antigen. (E) Elisa tests the affinity of different concentrations of BiKE to the <t>CD16A</t> antigen. (F) Indirect labeling Flow cytometry was used to assess the binding ability of BiKE to MM1.S, NK, and K562 cells. Goat anti human IgG (H + L) antibody labeled with FITC as the secondary antibody. (G) PBMCs were co-cultured with LILRB4 + MM1.S cells for 4 hours. The regulation of CD69 expression in NK cells by monoclonal antibodies and BiKE in the co-culture system was analyzed by flow cytometry. Data are presented as mean ± SD, *P < 0.05, ***P < 0.001, ****P < 0.0001, n = 4 compared to each other group by one-way ANOVA.
    Cd16a 6his Tagged Recombinant, supplied by TargetMol, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd16a+6his+tagged+recombinant/pmc12716741-76-7-10?v=TargetMol
    Average 93 stars, based on 2 article reviews
    cd16a 6his tagged recombinant - by Bioz Stars, 2026-08
    93/100 stars

    Images

    1) Product Images from "The combination of LILRB4-targeting NK cell engagers and cGAS–STING agonists enhances the anti–multiple myeloma immune activity of NK cells"

    Article Title: The combination of LILRB4-targeting NK cell engagers and cGAS–STING agonists enhances the anti–multiple myeloma immune activity of NK cells

    Journal: PLOS One

    doi: 10.1371/journal.pone.0339375

    (A) Schematic representation of the BiKE structure. (B) Schematic representation of BiKE-mediated NK cells killing of tumors. (C) BiKE SDS-PAGE of non-reduced and reduced proteins, stained with Coomassie blue. (D) ELISA test measuring the affinity of different concentrations of BiKE to the LILRB4 antigen. (E) Elisa tests the affinity of different concentrations of BiKE to the CD16A antigen. (F) Indirect labeling Flow cytometry was used to assess the binding ability of BiKE to MM1.S, NK, and K562 cells. Goat anti human IgG (H + L) antibody labeled with FITC as the secondary antibody. (G) PBMCs were co-cultured with LILRB4 + MM1.S cells for 4 hours. The regulation of CD69 expression in NK cells by monoclonal antibodies and BiKE in the co-culture system was analyzed by flow cytometry. Data are presented as mean ± SD, *P < 0.05, ***P < 0.001, ****P < 0.0001, n = 4 compared to each other group by one-way ANOVA.
    Figure Legend Snippet: (A) Schematic representation of the BiKE structure. (B) Schematic representation of BiKE-mediated NK cells killing of tumors. (C) BiKE SDS-PAGE of non-reduced and reduced proteins, stained with Coomassie blue. (D) ELISA test measuring the affinity of different concentrations of BiKE to the LILRB4 antigen. (E) Elisa tests the affinity of different concentrations of BiKE to the CD16A antigen. (F) Indirect labeling Flow cytometry was used to assess the binding ability of BiKE to MM1.S, NK, and K562 cells. Goat anti human IgG (H + L) antibody labeled with FITC as the secondary antibody. (G) PBMCs were co-cultured with LILRB4 + MM1.S cells for 4 hours. The regulation of CD69 expression in NK cells by monoclonal antibodies and BiKE in the co-culture system was analyzed by flow cytometry. Data are presented as mean ± SD, *P < 0.05, ***P < 0.001, ****P < 0.0001, n = 4 compared to each other group by one-way ANOVA.

    Techniques Used: SDS Page, Staining, Enzyme-linked Immunosorbent Assay, Labeling, Flow Cytometry, Binding Assay, Cell Culture, Expressing, Bioprocessing, Co-Culture Assay



    Similar Products

    cd16a 6his tagged recombinant  (TargetMol)
    93
    TargetMol cd16a 6his tagged recombinant
    (A) Schematic representation of the BiKE structure. (B) Schematic representation of BiKE-mediated NK cells killing of tumors. (C) BiKE SDS-PAGE of non-reduced and reduced proteins, stained with Coomassie blue. (D) ELISA test measuring the affinity of different concentrations of BiKE to the LILRB4 antigen. (E) Elisa tests the affinity of different concentrations of BiKE to the <t>CD16A</t> antigen. (F) Indirect labeling Flow cytometry was used to assess the binding ability of BiKE to MM1.S, NK, and K562 cells. Goat anti human IgG (H + L) antibody labeled with FITC as the secondary antibody. (G) PBMCs were co-cultured with LILRB4 + MM1.S cells for 4 hours. The regulation of CD69 expression in NK cells by monoclonal antibodies and BiKE in the co-culture system was analyzed by flow cytometry. Data are presented as mean ± SD, *P < 0.05, ***P < 0.001, ****P < 0.0001, n = 4 compared to each other group by one-way ANOVA.
    Cd16a 6his Tagged Recombinant, supplied by TargetMol, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd16a+6his+tagged+recombinant/pmc12716741-76-7-10?v=TargetMol
    Average 93 stars, based on 1 article reviews
    cd16a 6his tagged recombinant - by Bioz Stars, 2026-08
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    (A) Schematic representation of the BiKE structure. (B) Schematic representation of BiKE-mediated NK cells killing of tumors. (C) BiKE SDS-PAGE of non-reduced and reduced proteins, stained with Coomassie blue. (D) ELISA test measuring the affinity of different concentrations of BiKE to the LILRB4 antigen. (E) Elisa tests the affinity of different concentrations of BiKE to the CD16A antigen. (F) Indirect labeling Flow cytometry was used to assess the binding ability of BiKE to MM1.S, NK, and K562 cells. Goat anti human IgG (H + L) antibody labeled with FITC as the secondary antibody. (G) PBMCs were co-cultured with LILRB4 + MM1.S cells for 4 hours. The regulation of CD69 expression in NK cells by monoclonal antibodies and BiKE in the co-culture system was analyzed by flow cytometry. Data are presented as mean ± SD, *P < 0.05, ***P < 0.001, ****P < 0.0001, n = 4 compared to each other group by one-way ANOVA.

    Journal: PLOS One

    Article Title: The combination of LILRB4-targeting NK cell engagers and cGAS–STING agonists enhances the anti–multiple myeloma immune activity of NK cells

    doi: 10.1371/journal.pone.0339375

    Figure Lengend Snippet: (A) Schematic representation of the BiKE structure. (B) Schematic representation of BiKE-mediated NK cells killing of tumors. (C) BiKE SDS-PAGE of non-reduced and reduced proteins, stained with Coomassie blue. (D) ELISA test measuring the affinity of different concentrations of BiKE to the LILRB4 antigen. (E) Elisa tests the affinity of different concentrations of BiKE to the CD16A antigen. (F) Indirect labeling Flow cytometry was used to assess the binding ability of BiKE to MM1.S, NK, and K562 cells. Goat anti human IgG (H + L) antibody labeled with FITC as the secondary antibody. (G) PBMCs were co-cultured with LILRB4 + MM1.S cells for 4 hours. The regulation of CD69 expression in NK cells by monoclonal antibodies and BiKE in the co-culture system was analyzed by flow cytometry. Data are presented as mean ± SD, *P < 0.05, ***P < 0.001, ****P < 0.0001, n = 4 compared to each other group by one-way ANOVA.

    Article Snippet: For antibody affinity testing, LILRB4 (hFc-tagged) and CD16A (6His-tagged) recombinant proteins(TargetMol, USA) were coated onto 96-well plates at 50 ng/well and incubated overnight at 4°C.

    Techniques: SDS Page, Staining, Enzyme-linked Immunosorbent Assay, Labeling, Flow Cytometry, Binding Assay, Cell Culture, Expressing, Bioprocessing, Co-Culture Assay